Monoclonal antibody to cytokeratin for use in routine histopathology.
نویسندگان
چکیده
CAM 5.2 is a murine monoclonal antibody, raised against the colon carcinoma cell line HT29, which recognises lower molecular weight intracellular cytokeratin proteins within secretory epithelia. Extensive indirect immunohistochemical studies have confirmed that this antibody stains formalin fixed (and freshly frozen) normal and malignant human tissue in a consistent manner. Reliable staining of conventionally processed pathological tissues provides more accurate identification and staging of human malignant epithelial diseases.
منابع مشابه
Immunohistochemical Characterization of Normal Ovary and Common Epithelial Ovarian Neoplasm with a Monoclonal Antibody to Cytokeratin and Vimentin
Background & Objective: The common epithelial ovarian tumors are classified into serous, mucinous, clear cell, endometrioid, the Brenner, mixed, and undifferentiated types. Cytoskeleton intermediate filament composition of ovarian tissues indicates that the cytokeratin and vimentin are observed in ovarian surface epithelium along with the common ovaria...
متن کاملIdentification of Malignant Cells in Serous Fluids Using a Panel of Monoclonal Cytokeratin Antibodies, Epithelial Membrane Antigen(EMA) , Carcino Embryonic Antigen (CEA)
Abstract Background and Objective: Identification of malignant cells and the type of malignancy in Effusionsis very important. The main aim of this study was to differentiate between reactive mesothelial cells and malignant cells and to determine the type of the tumor cells in effusions with the aid of tumor markers Creatine Kinase (CK), EMA and CEA. Material and Methods: Forty serous flui...
متن کاملExpression of cytokeratin and neuron-specific enolase in small cell carcinomas of the lung.
Using a polyclonal antibody against human epidermal keratins and a monoclonal antibody against cytokeratins characteristic of simple epithelia, and the Avidin-Biotin system of immunohistochemistry, we have demonstrated cytokeratin expression in 46% and in 60% of small cell carcinomas of the lung at autopsy respectively. The latter gave a diffuse stronger reaction product than the polyclonal ant...
متن کاملComparing the Diagnostic Outcomes of Staining Various Breast Lesions with Either Anti-CK 5/6 or Anti-CK 5
Background and Objective: Anti-CK5/6 monoclonal antibodies have an established role in breast disease diagnosis. Anti-CK5 monoclonal antibodies have recently become commercially available. There has been growing interest in the staining characteristics of anti-CK5 and its potential diagnostic role in place of anti-CK5/6. We aim to compare and contrast the staining characteristi...
متن کاملComparison of Expression of Cytokeratin 7 on Epithelium of Nasal Polyps and Mucosa of Chronic Rhinosinusitis
Background and Objective: Nasal polyp is the most common benign mass of the nasal cavity and it is originated from nasal cavity or sinuses. Cytokeratins (CK) are intermediate filament proteins that provide mechanical support in epithelial cells. The purpose of this study was to compare the expression of cytokeratin 7 in epithelium of nasal polyps and nasal mucosa in chronic rhinosinusitis. Mate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of clinical pathology
دوره 37 9 شماره
صفحات -
تاریخ انتشار 1984